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Boster Bio
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GeneTex
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OriGene
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Image Search Results
Journal: Frontiers in Psychiatry
Article Title: Histone deacetylase inhibitors mitigate antipsychotic risperidone-induced motor side effects in aged mice and in a mouse model of Alzheimer’s disease
doi: 10.3389/fpsyt.2022.1020831
Figure Lengend Snippet: Effects of histone deacetylase (HDAC) inhibitors on histone acetylation at the 5htr2a and Drd2 gene promoters in the striatum in young and aged mice. (A) No significant differences of H3K27ac binding at the 5htr2a promoter between young and aged group was found, but a significant increase in H3K27ac binding at the 5ht2a promoter in the striatum was found in aged and young mice co-treated with RIS and VPA or MS-275 compared to those treated with RIS alone. (B) H3K27ac binding at the Drd2 promoter was significantly decreased in aged mice as compared to young mice in Veh treated group. However, H3K27ac binding at the Drd2 promoter in the striatum was observed to be significantly increased in aged mice co-treated with RIS and HDAC inhibitors VPA or MS-275 when compared to those treated with Veh alone. Data are presented as mean ± SEM, ( n = 5). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
Article Snippet: Blots were blocked for 1-hour with 5% non-fat dry milk (Biorad, 1706404) and incubated using 1:1,000
Techniques: Histone Deacetylase Assay, Binding Assay
Journal: Ecotoxicology and environmental safety
Article Title: Chronic developmental exposure to low-dose ([C8mim][PF6]) induces neurotoxicity and behavioural abnormalities in rats.
doi: 10.1016/j.ecoenv.2021.112806
Figure Lengend Snippet: Fig. 4. Developmental exposure to low-dose ([C8MIM] [PF6]) increased the expression of NMDA receptors. (A) The mRNA level of major receptors (nmdar1, nmdar2a, nmdar2b for NMDA receptor; drd1 and drd2 for dopamine receptor; 5-ht2ar for 5-HT receptor; gabaarα1 for GABA receptor) for main neurotransmitters in hippocampus and prefrontal cortex. (B) The protein levels of NMDA receptors (NMDAR1, NMDAR2A, and NMDAR2B) in the hippocam pus and prefrontal cortex. (C) The protein levels of dopa mine receptors (DRD1 and DRD2), 5-HT receptors (5- HT2AR), and GABA receptors (GABAARα1) in the hippo campus and prefrontal cortex. (D) Statistical analysis of protein expression. n = 3 for each group. Student’s t-test was performed for the analysis. The data are presented as the mean ± SEM. *p<0.05, **p<0.01, ***p<0.001.
Article Snippet: Membranes were treated with 5% skim milk for 1 h, followed by incubation with primary antibodies against NMDAR1 (ab109182, Abcam), NMDAR2A (ab124913, Abcam), NMDAR2B (ab65783, Abcam), DRD1 (ab81296, Abcam), DRD2 (ab30743, Abcam),
Techniques: Expressing
Journal: Journal of Diabetes Investigation
Article Title: Important role of 5‐hydroxytryptamine in glucocorticoid‐induced insulin resistance in liver and intra‐abdominal adipose tissue of rats
doi: 10.1111/jdi.12406
Figure Lengend Snippet: Primers used for reverse transcription polymerase chain reaction
Article Snippet: After being blocked, the membranes were then incubated with appropriate primary antibodies: Tph1 and AADC (Epitomics‐Abcam, Hong Kong), PEPCK‐1 (Signalway Antibody LLC, College Park, MD USA), GPAT1 (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), GLUT2 (Epitomics‐Abcam), 5‐HTR 2A,
Techniques: Reverse Transcription